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Regulatory Mechanism of EAAC1 Expression in Neurons While GLAST and GLT-1 are constitutively expressed on the cell membrane of glial cells, the membrane expression levels of EAAC1 are approximately 20% of the total under normal conditions, while protein kinase C (PKC) and phosphoinositide 3-kinase (PI3K) activations increase the EAAC1 expression on the plasma membrane [56] (Figure 3)